mg 132 proteasome inhibitor Search Results


proteasome inhibitor mg-132  (CH Instruments)
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proteasome inhibitor mg-132  (Merck & Co)
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Merck & Co proteasome inhibitor mg-132
Proteasome Inhibitor Mg 132, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10µm mg-132 proteasome inhibitor  (Merck & Co)
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mg-132  (Merck & Co)
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Merck & Co mg-132
SMURF2 positively regulates KAP1 protein levels in tumor cells. ( A ) Western blot analysis showing that overexpression of SMURF2WT, but not its E3 ligase-inactive mutant (SMURF2 CG ), enhances the protein abundance of KAP1 in osteosarcoma U2OS cells. ( B ) Immunoblot analysis showing diminished protein levels of KAP1 in SMURF2 knockdown U2OS cells. Two different siRNAs targeting SMURF2 mRNA expression at either 3′UTR or coding sequence (CDS) were used. NS—non-silencing siRNA. ( C ) Immunoblot analysis of KAP1 expression in SMURF2 CRISPR knockdown U2OS cells ( SMURF2 CR , left panels) and in cells following SMURF2 reconstitution (right panels). SMURF2 CR cells were reconstituted with FLAG-SMURF2. Cells transduced with an empty FLAG vector were used as a control. ( D ) Western blot analysis showing the effect of SMURF2 knockdown on KAP1 protein levels in different types of human cancer cells: cervix carcinoma HeLa cells, prostate carcinoma DU-145 cells, and breast carcinoma MDA-MB-468 cells. Non-silencing siNS and shLuc were used as controls for siRNA and shRNA experiments, respectively. ( E , F ) Confocal microscopy analysis of KAP1 expression in SMURF2 knockdown MDA-MB-468 cells. Scale bars: 20 µm. Quantification of the results (panel F) is shown as mean ± SD. n—number of cells quantified for each group from 10 different fields. ( G ) Western blot analysis of KAP1 protein expression in SMURF2 knockdown U2OS cells treated with the proteasomal inhibitor <t>MG-132</t> (5 µM; 4 h). The inhibition of the proteasomal pathway by MG-132 was verified using anti-poly-ubiquitin-K48-specific antibody. ( H ) Quantification of data shown in ( G ) from two independent experiments. Data are mean ± SD. The uncropped Western blot images can be found in .
Mg 132, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mg-132/product/Merck & Co
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mg-132 (catalogue number c2211)  (Merck & Co)
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Merck & Co mg-132 (catalogue number c2211)
SMURF2 positively regulates KAP1 protein levels in tumor cells. ( A ) Western blot analysis showing that overexpression of SMURF2WT, but not its E3 ligase-inactive mutant (SMURF2 CG ), enhances the protein abundance of KAP1 in osteosarcoma U2OS cells. ( B ) Immunoblot analysis showing diminished protein levels of KAP1 in SMURF2 knockdown U2OS cells. Two different siRNAs targeting SMURF2 mRNA expression at either 3′UTR or coding sequence (CDS) were used. NS—non-silencing siRNA. ( C ) Immunoblot analysis of KAP1 expression in SMURF2 CRISPR knockdown U2OS cells ( SMURF2 CR , left panels) and in cells following SMURF2 reconstitution (right panels). SMURF2 CR cells were reconstituted with FLAG-SMURF2. Cells transduced with an empty FLAG vector were used as a control. ( D ) Western blot analysis showing the effect of SMURF2 knockdown on KAP1 protein levels in different types of human cancer cells: cervix carcinoma HeLa cells, prostate carcinoma DU-145 cells, and breast carcinoma MDA-MB-468 cells. Non-silencing siNS and shLuc were used as controls for siRNA and shRNA experiments, respectively. ( E , F ) Confocal microscopy analysis of KAP1 expression in SMURF2 knockdown MDA-MB-468 cells. Scale bars: 20 µm. Quantification of the results (panel F) is shown as mean ± SD. n—number of cells quantified for each group from 10 different fields. ( G ) Western blot analysis of KAP1 protein expression in SMURF2 knockdown U2OS cells treated with the proteasomal inhibitor <t>MG-132</t> (5 µM; 4 h). The inhibition of the proteasomal pathway by MG-132 was verified using anti-poly-ubiquitin-K48-specific antibody. ( H ) Quantification of data shown in ( G ) from two independent experiments. Data are mean ± SD. The uncropped Western blot images can be found in .
Mg 132 (Catalogue Number C2211), supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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proteasome inhibitor mg–132  (Axon Medchem LLC)
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Axon Medchem LLC proteasome inhibitor mg–132
SMURF2 positively regulates KAP1 protein levels in tumor cells. ( A ) Western blot analysis showing that overexpression of SMURF2WT, but not its E3 ligase-inactive mutant (SMURF2 CG ), enhances the protein abundance of KAP1 in osteosarcoma U2OS cells. ( B ) Immunoblot analysis showing diminished protein levels of KAP1 in SMURF2 knockdown U2OS cells. Two different siRNAs targeting SMURF2 mRNA expression at either 3′UTR or coding sequence (CDS) were used. NS—non-silencing siRNA. ( C ) Immunoblot analysis of KAP1 expression in SMURF2 CRISPR knockdown U2OS cells ( SMURF2 CR , left panels) and in cells following SMURF2 reconstitution (right panels). SMURF2 CR cells were reconstituted with FLAG-SMURF2. Cells transduced with an empty FLAG vector were used as a control. ( D ) Western blot analysis showing the effect of SMURF2 knockdown on KAP1 protein levels in different types of human cancer cells: cervix carcinoma HeLa cells, prostate carcinoma DU-145 cells, and breast carcinoma MDA-MB-468 cells. Non-silencing siNS and shLuc were used as controls for siRNA and shRNA experiments, respectively. ( E , F ) Confocal microscopy analysis of KAP1 expression in SMURF2 knockdown MDA-MB-468 cells. Scale bars: 20 µm. Quantification of the results (panel F) is shown as mean ± SD. n—number of cells quantified for each group from 10 different fields. ( G ) Western blot analysis of KAP1 protein expression in SMURF2 knockdown U2OS cells treated with the proteasomal inhibitor <t>MG-132</t> (5 µM; 4 h). The inhibition of the proteasomal pathway by MG-132 was verified using anti-poly-ubiquitin-K48-specific antibody. ( H ) Quantification of data shown in ( G ) from two independent experiments. Data are mean ± SD. The uncropped Western blot images can be found in .
Proteasome Inhibitor Mg–132, supplied by Axon Medchem LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ubiquitin proteasome inhibitor mg-132  (Pfizer Inc)
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Pfizer Inc ubiquitin proteasome inhibitor mg-132
SMURF2 positively regulates KAP1 protein levels in tumor cells. ( A ) Western blot analysis showing that overexpression of SMURF2WT, but not its E3 ligase-inactive mutant (SMURF2 CG ), enhances the protein abundance of KAP1 in osteosarcoma U2OS cells. ( B ) Immunoblot analysis showing diminished protein levels of KAP1 in SMURF2 knockdown U2OS cells. Two different siRNAs targeting SMURF2 mRNA expression at either 3′UTR or coding sequence (CDS) were used. NS—non-silencing siRNA. ( C ) Immunoblot analysis of KAP1 expression in SMURF2 CRISPR knockdown U2OS cells ( SMURF2 CR , left panels) and in cells following SMURF2 reconstitution (right panels). SMURF2 CR cells were reconstituted with FLAG-SMURF2. Cells transduced with an empty FLAG vector were used as a control. ( D ) Western blot analysis showing the effect of SMURF2 knockdown on KAP1 protein levels in different types of human cancer cells: cervix carcinoma HeLa cells, prostate carcinoma DU-145 cells, and breast carcinoma MDA-MB-468 cells. Non-silencing siNS and shLuc were used as controls for siRNA and shRNA experiments, respectively. ( E , F ) Confocal microscopy analysis of KAP1 expression in SMURF2 knockdown MDA-MB-468 cells. Scale bars: 20 µm. Quantification of the results (panel F) is shown as mean ± SD. n—number of cells quantified for each group from 10 different fields. ( G ) Western blot analysis of KAP1 protein expression in SMURF2 knockdown U2OS cells treated with the proteasomal inhibitor <t>MG-132</t> (5 µM; 4 h). The inhibition of the proteasomal pathway by MG-132 was verified using anti-poly-ubiquitin-K48-specific antibody. ( H ) Quantification of data shown in ( G ) from two independent experiments. Data are mean ± SD. The uncropped Western blot images can be found in .
Ubiquitin Proteasome Inhibitor Mg 132, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


SMURF2 positively regulates KAP1 protein levels in tumor cells. ( A ) Western blot analysis showing that overexpression of SMURF2WT, but not its E3 ligase-inactive mutant (SMURF2 CG ), enhances the protein abundance of KAP1 in osteosarcoma U2OS cells. ( B ) Immunoblot analysis showing diminished protein levels of KAP1 in SMURF2 knockdown U2OS cells. Two different siRNAs targeting SMURF2 mRNA expression at either 3′UTR or coding sequence (CDS) were used. NS—non-silencing siRNA. ( C ) Immunoblot analysis of KAP1 expression in SMURF2 CRISPR knockdown U2OS cells ( SMURF2 CR , left panels) and in cells following SMURF2 reconstitution (right panels). SMURF2 CR cells were reconstituted with FLAG-SMURF2. Cells transduced with an empty FLAG vector were used as a control. ( D ) Western blot analysis showing the effect of SMURF2 knockdown on KAP1 protein levels in different types of human cancer cells: cervix carcinoma HeLa cells, prostate carcinoma DU-145 cells, and breast carcinoma MDA-MB-468 cells. Non-silencing siNS and shLuc were used as controls for siRNA and shRNA experiments, respectively. ( E , F ) Confocal microscopy analysis of KAP1 expression in SMURF2 knockdown MDA-MB-468 cells. Scale bars: 20 µm. Quantification of the results (panel F) is shown as mean ± SD. n—number of cells quantified for each group from 10 different fields. ( G ) Western blot analysis of KAP1 protein expression in SMURF2 knockdown U2OS cells treated with the proteasomal inhibitor MG-132 (5 µM; 4 h). The inhibition of the proteasomal pathway by MG-132 was verified using anti-poly-ubiquitin-K48-specific antibody. ( H ) Quantification of data shown in ( G ) from two independent experiments. Data are mean ± SD. The uncropped Western blot images can be found in .

Journal: Cancers

Article Title: The Emerging Role of E3 Ubiquitin Ligase SMURF2 in the Regulation of Transcriptional Co-Repressor KAP1 in Untransformed and Cancer Cells and Tissues

doi: 10.3390/cancers14071607

Figure Lengend Snippet: SMURF2 positively regulates KAP1 protein levels in tumor cells. ( A ) Western blot analysis showing that overexpression of SMURF2WT, but not its E3 ligase-inactive mutant (SMURF2 CG ), enhances the protein abundance of KAP1 in osteosarcoma U2OS cells. ( B ) Immunoblot analysis showing diminished protein levels of KAP1 in SMURF2 knockdown U2OS cells. Two different siRNAs targeting SMURF2 mRNA expression at either 3′UTR or coding sequence (CDS) were used. NS—non-silencing siRNA. ( C ) Immunoblot analysis of KAP1 expression in SMURF2 CRISPR knockdown U2OS cells ( SMURF2 CR , left panels) and in cells following SMURF2 reconstitution (right panels). SMURF2 CR cells were reconstituted with FLAG-SMURF2. Cells transduced with an empty FLAG vector were used as a control. ( D ) Western blot analysis showing the effect of SMURF2 knockdown on KAP1 protein levels in different types of human cancer cells: cervix carcinoma HeLa cells, prostate carcinoma DU-145 cells, and breast carcinoma MDA-MB-468 cells. Non-silencing siNS and shLuc were used as controls for siRNA and shRNA experiments, respectively. ( E , F ) Confocal microscopy analysis of KAP1 expression in SMURF2 knockdown MDA-MB-468 cells. Scale bars: 20 µm. Quantification of the results (panel F) is shown as mean ± SD. n—number of cells quantified for each group from 10 different fields. ( G ) Western blot analysis of KAP1 protein expression in SMURF2 knockdown U2OS cells treated with the proteasomal inhibitor MG-132 (5 µM; 4 h). The inhibition of the proteasomal pathway by MG-132 was verified using anti-poly-ubiquitin-K48-specific antibody. ( H ) Quantification of data shown in ( G ) from two independent experiments. Data are mean ± SD. The uncropped Western blot images can be found in .

Article Snippet: The proteasomal inhibitor MG-132 and deubiquitinase (DUB) inhibitor N-ethylmaleimide (NEM) were purchased from Merck.

Techniques: Western Blot, Over Expression, Mutagenesis, Expressing, Sequencing, CRISPR, Transduction, Plasmid Preparation, shRNA, Confocal Microscopy, Inhibition